ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (11): 1844-1850.doi: 10.11843/j.issn.0366-6964.2014.11.015

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Genetic Analysis of the Entire Genome of One H12N7 Subtype Avian Influenza Virus

TAN Dan1,2,CUI Peng-fei3,HUANG Jian-long1,WANG Chang-jian1,LI Wen-hui3,FAN Zhong-xin1,ZHANG Fang3,HE Shi-cheng1,TANG Xiao-ming1,LIU Dao-xin1*,DENG Guo-hua3*   

  1. (1.Hunan Provincial Animal Disease Prevention and Control Center,Changsha 410014,China;2.Changsha Animal Disease Prevention and Control Center,Changsha 410013,China;3.Animal Influenza Laboratory of the Ministry of Agriculture,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China)
  • Received:2014-03-24 Online:2014-11-23 Published:2014-11-23

Abstract:

H12N7 subtype avian influenza virus (AIV) [A/Environment /Hunan/S4484/2011(H12N7)] was isolated from a duck farm environment in Dongting lake of Hunan during the routine epidemiologic surveillance in 2011.The sequence analysis of the hemagglutinin (HA) gene showed that the virus was categorized as low pathogenic AIV,with the typical HA cleavage site of PQAQDR↓GLF.The phylogenic analysis indicated that all 8 segments of A/Environment /Hunan/S4484/2011(H12N7) was derived from the Eurasian lineage,but the source of the 8 gene segments was very complex,and the highly homologous was observed with different subtypes of AIVs in some regions in Asia and Europe in varying degrees and extensively.Our results suggested that the virus may be a multi-origin reassortant virus.

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